Effects of histone hypomethylation induced by alcohol during pregnancy on overexpression of cardiomyogenesis genes offspring mice

AIM:To investigate the effects of histone methylation on the abnormal expression of cardiomyogenesis genes caused by alcohol during pregnancy and the regulatory mechanism, and to provide a new idea and intervention targets for preventing and curing congenital heart disease. METHODS:The alcohol (56%, 5 mL/kg) and G9a-histone methyltransferases (HMT) inhibitor BRD4770 (1 mg/kg) were given by gavage in Kunming mice during embryo (E) 0.5~14.5 d, and the hearts of the mice in E14.5, E16.5 and post neonatal 0.5 d (PND0.5) were collected. The mRNA expression of Gata4, Cx43 and β-MHC genes was detected by RT-qPCR. The activity of HMT was measured by colorimetry. Meanwhile, the protein expression of histone H3K9me3, G9a-HMT, Cx43 and β-MHC was determined by Western blot. RESULTS:The results of colorimetry showed that the activity of HMT in the heart of the offspring mice treated with alcohol during pregnancy was decreased significantly compared with normal saline group (P<0.05), and Western blot data showed that the expression of G9a-HMT and histone H3K9me3 were apparently decreased in the same samples (P<0.05). The mRNA expression levels of Gata4, Cx43 and β-MHC in alcohol group were apparently increased compared with normal saline group (P<0.05). Meanwhile, the protein levels of Cx43 and β-MHC were increased significantly in the same samples (P<0.05). However, BRD4770, a G9a-HMT inhibitor, further attenuated the level of histone H3K9me3, and further upregulated the expression of Gata4, Cx43 and β-MHC in the heart of the the mice treated with alcohol (P<0.05). CONCLUSION:Histone methylation modification imbalance induced by G9a-HMT may be involved in the abnormal expression of cardiomyogenesis genes in the heart of offspring mice caused by alcohol during pregnancy.     

东北黑土区坡耕地斜坡垄作与顺坡垄作土壤侵蚀的对比分析

斜坡垄作是东北黑土区最普遍的垄作方式之一，但当前关于斜坡垄作对坡耕地土壤侵蚀的影响鲜见报道。为此，本研究基于室内模拟试验，设计2个降雨强度（50和100 mm/h）以及2种垄作方式（斜坡垄作和顺坡垄作），分析东北黑土区坡耕地斜坡垄作与顺坡垄作坡面土壤侵蚀的差异。结果表明：（1）在50和100 mm/h降雨强度下，斜坡垄作断垄前坡面侵蚀速率分别是顺坡垄作的0.46%和0.35%；但在45 min的降雨过程中，由于斜坡垄作发生断垄现象，造成50和100 mm/h两种降雨强度下斜坡垄作坡面侵蚀速率分别是顺坡垄作的1.24和1.03倍。（2）斜坡垄作径流强度和侵蚀速率随降雨历时的变化均从断垄开始发生突变。在50和100 mm/h降雨强度下，随着降雨历时的变化斜坡垄作断垄前的径流强度和侵蚀速率值均低于顺坡垄作,其平均径流强度分别为顺坡垄作的8.42%和3.75%、平均侵蚀速率分别为顺坡垄作的0.46%和0.35%，但斜坡垄作断垄后坡面径流和侵蚀速率明显增大，其平均径流强度分别为顺坡垄作的1.33和1.47倍、平均侵蚀速率分别是顺坡垄作的2.03和1.62倍。（3）在50和100 mm/h降雨强度下，斜坡垄作断垄前坡面径流量和侵蚀量存在显著的线性关系（P<0.01），而断垄后两者的相关关系则不显著（P>0.05）；而顺坡垄作在两种降雨强度下坡面径流量和侵蚀量存在显著的线性关系。（4）在两种降雨强度下斜坡垄作坡面90%以上的径流泥沙均来自断垄后。因此，提高垄丘稳定性和防止断垄现象发生，是减少斜坡垄作坡面土壤侵蚀的关键所在。     

美国油气管道基本失效概率评估方法及启示

为充分利用油气管道历史失效数据,减少失效概率评估过程中的主观性,提出油气管道基本失效概率的概念及评估方法。对美国管道及危险物品安全管理局数据库的油气管道里程数据、事故数据及失效因素数据进行全面分析,采用基于事故统计的方法评估油气管道基本失效概率。结果表明:美国危险液体管道、输气集气管道、配气管道发生一般事故的基本失效概率分别为1.29次/(10^3 km·a)、2.17次/(10^4 km·a)及4.08次/(10^5 km·a),发生较大事故的基本失效概率分别为4.58次/(10^4 km·a)、1.41次/(10^4 km·a)及2.38次/(10^5 km·a),发生重大事故的基本失效概率分别为9.09次/(10^6 km·a)、9.79次/(10^6 km·a)及1.11次/(10^5 km·a)。美国基本失效概率可作为油气管道失效概率评估的基准线,也可作为风险可接受标准的依据,但不能直接应用于中国管道风险评价,需对其进行修正,建立适用于中国管道的失效数据库。     

基于USLE模型的长株潭城市群生态绿心区水土流失研究

[目的]研究2018年长株潭(长沙市—株州市—湘潭市)城市群生态绿心区最佳植被覆盖和水土流失状况,为区域综合治理提供理论依据。[方法]运用GIS和RS技术,以长株潭区域降雨、土壤、地形、土地利用等数据为基础,采用归一化植被指数(NDVI)模型和USLE国际通用土壤流失方程为优选模型。[结果]长株潭绿心区总面积为52 287 hm~2,整体植被状况较好,高覆盖度(75%～100%)面积最大,占绿心区总面积一半以上,为26 598.40 hm~2;中低覆盖度(30%～40%)面积最小,占区域总面积的8.61%,为4 501.91 hm~2。长株潭城市群生态绿心区总侵蚀(不含微度)面积为3 654.24 hm~2,占总面积的6.99%。湘潭市侵蚀比重最高,为8.51%,长沙市次之,为6.67%,株洲市侵蚀总比例最小,为5.68%。工程建设用地在禁止开发区、限制开发区和控制建设区的侵蚀面积分别为963.92,310.74,735.11 hm~2。[结论]受人为因素、城市建设、产业分布等影响,长株潭城市群生态绿心区覆盖度空间呈现西部低,中东部高的格局,工程建设是造成绿心区土壤侵蚀的主要原因。     

空气罐与超压泄压阀联合水锤防护特性

提出了一种空气罐与超压泄压阀联合设置的停泵水锤防护方案,并基于瞬变流计算的特征线法建立了空气罐、液控蝶阀、超压泄压阀等边界条件的数学模型,模拟了停泵工况下系统的压力变化过程.结合工程实例,对比分析了空气罐单独防护、空气罐与两阶段关闭泵后液控蝶阀联合防护以及空气罐与超压泄压阀联合防护对停泵水锤的影响.在空气罐体型一定时,对超压泄压阀的启闭规律进行了敏感性分析.计算结果表明,空气罐与两阶段关闭泵后液控蝶阀联合防护方案对输水系统正负水锤防护均不利;而空气罐与超压泄压阀联合防护方案对输水系统正负水锤均有较好的防护效果,与空气罐单独防护方案相比,泵后高压管段最高压应力由1.343 MPa降至1.087 MPa,在满足承压标准1.2 MPa的基础上安全裕度提高了9.4%,空气罐体型也由200 m³缩减至160 m³.超压泄压阀应在5 s内开启至全开度,且开启后持续时间应接近1个相长.     

Effects of cardiac contractility modulation on ventricular electrical remodeling in rabbits with chronic heart failure

AIM: To investigate the effects of cardiac contractility modulation (CCM) on ventricular electrical remodeling in a rabbit model of chronic heart failure. METHODS: The rabbits were divided into sham group, heart failure(HF) group and HF+ CCM group. The rabbit model of chronic heart failure was established by ligating the ascending aortic root. Then electrical stimulations during the absolute refractory period were delivered lasting 6 h everyday for 4 weeks in rabbits of HF+ CCM group. The QTc and ventricular effective refrective period (VERP) were recorded. The protein and mRNA levels of Kv1.4, Kv4.3 and connexin 43 (Cx43) were determined by Western blot and RT-qPCR. RESULTS: Compared with sham group, QTc were significantly prolonged in HF rabbits at week 12 (P<0.05). CCM therapy shortened QTc of rabbits with heart failure at week 16 (P<0.05). Compared with sham group, VERP significantly increased in HF group and HF+ CCM group, while CCM therapy shortened VERP of rabbits with heart failure at week 16 (P<0.05). Compared with sham group, the mRNA and protein levels of Kv1.4, Kv4.3 and Cx43 were decreased in HF group and HF+ CCM group (P<0.05). However, CCM therapy restored the mRNA and protein levels of Kv1.4, Kv4.3 and Cx43 of rabbits with heart failure (P<0.05). CONCLUSION: CCM suppresses ventricular electrical remodeling in heart failure and the underlying mechanism may be associated with increasing Kv1.4, Kv4.3 and Cx43 expression.     

基于热致液晶弹性体的仿生心脏泵的设计

为设计一款仿生心脏功能较佳的心室泵,采用柔性、热致伸缩的热致形变弹性体代替心肌,根据螺旋心室心肌带理论,选择心肌带的左心室段,并选取了Hilbert平面填充曲线作为热致导体在热致形变弹性体中的走行方式,设计出与真实心脏类似的心室泵结构.通过试验测量了所设计的心室泵的射血分数与心率,结果显示该心室泵的搏出量高于自然心脏,而其心率远低于自然心脏.相对于机械泵,所设计的心室泵结构与真实心脏类似,具有收缩能力强,射血分数高的优点,且不易破坏血细胞,生物相容性较好.不足之处在于,热致动液晶弹性体变形响应慢,暂时还未能达到正常心脏的心率.论证了构建双稳态结构,提高仿生心室泵效率的可能性,以及对于心肌纤维走向较为复杂的其他心肌带段,双轴拉伸机械雕刻对其仿生的可能性.此研究可为容积心脏泵的研发提供新的思路,也可以对右心衰竭等心脏疾病起到辅助治疗的作用.     

“四全”育人夯实立德树人德育基础——以苏州工业职业技术学院为例

随着社会发展的推动和各种思想文化的交流、交融与交锋,给高职院校的思想政治教育工作带来前所未有的挑战。苏州工业职业技术学院积极探索实践"全员、全程、全方位、全心全意"育人的德育体系,在立德树人背景下,以踏实、务实、求实的作风不断夯实德育基础,努力开创"责任到人,纵向到底,横向到边"的德育工作局面。     

益肾降浊胶囊对腺嘌呤致大鼠慢性肾功能衰竭的影响研究

目的探讨益肾降浊胶囊对腺嘌呤诱导的慢性肾衰大鼠的肾脏保护作用。方法采用灌胃给与腺嘌呤法建立大鼠慢性肾衰(CRF)模型,造模后每日1次连续灌胃给予益肾降浊胶囊。4周后,测定各组大鼠24h尿量、24h尿蛋白含量,称取大鼠体质量、肾重并计算肾指数,测定大鼠血清中肌酐(Scr)及尿素氮(BUN)含量,并在光镜下观察肾脏组织变化情况。结果与对照组相比,模型组大鼠生长构建期间生长状态明显变差,体重增长缓慢。与CRF模型组大鼠相比,益肾降浊胶囊给药组的大鼠体重增加明显,大鼠血清中Scr、BUN及尿液中24h尿蛋白水平显著降低。病理分析结果显示,肾脏组织结构的紊乱、肾小球固缩,炎性细胞浸润、肾间质纤维化症状明显改善。结论益肾降浊胶囊能够有效的治疗腺嘌呤所致的大鼠慢性肾功能衰竭。